| 01 | NUFFIELD HEALTH | 19,361 | £1.45bn |
| 02 | SAVE THE CHILDREN INTERNATIONAL | 16,153 | £1.10bn |
| 03 | United Learning LTD | 11,294 | £790m |
| 04 | THE HALO TRUST | 11,216 | £166m |
| 05 | THE BRITISH COUNCIL | 9,446 | £1.01bn |
| 06 | THE NATIONAL TRUST FOR PLACES OF HISTORIC INTEREST OR NATURAL BEAUTY | 8,746 | £766m |
| 07 | MSI REPRODUCTIVE CHOICES | 8,724 | £310m |
| 08 | BARNARDO'S | 7,404 | £340m |
| 09 | ROYAL MENCAP SOCIETY | 7,394 | £244m |
| 10 | COMMUNITY INTEGRATED CARE | 6,443 | £197m |
| 11 | THE ORMISTON TRUST | 6,196 | £437m |
| 12 | CARDIFF UNIVERSITY | 6,092 | £634m |
| 13 | THE MINES ADVISORY GROUP | 5,809 | £99.3m |
| 14 | CHANGE, GROW, LIVE | 5,715 | £340m |
| 15 | CANCER RESEARCH UK | 5,169 | £735m |
| 16 | THE SHAW TRUST LIMITED | 4,998 | £291m |
| 17 | OASIS CHARITABLE TRUST | 4,978 | £344m |
| 18 | THE GIRLS' DAY SCHOOL TRUST | 4,726 | £373m |
| 19 | METHODIST HOMES | 4,698 | £283m |
| 20 | BRITISH HEART FOUNDATION | 4,545 | £411m |
| 21 | ACTION FOR CHILDREN | 4,395 | £163m |
| 22 | LLOYD'S REGISTER FOUNDATION | 4,163 | £692m |
| 23 | OXFAM | 4,084 | £339m |
| 24 | ST ANDREW'S HEALTHCARE | 4,081 | £236m |
| 25 | THE ORDERS OF ST JOHN CARE TRUST | 3,834 | £234m |
| 26 | MARIE CURIE | 3,582 | £181m |
| 27 | TURNING POINT | 3,521 | £192m |
| 28 | SWANSEA UNIVERSITY | 3,448 | £326m |
| 29 | UNITED RESPONSE | 3,358 | £107m |
| 30 | THERA TRUST | 3,346 | £101m |
| 31 | THE BRITISH RED CROSS SOCIETY | 3,286 | £287m |
| 32 | Waythrough | 3,183 | £165m |
| 33 | SENSE, THE NATIONAL DEAFBLIND AND RUBELLA ASSOCIATION | 2,956 | £102m |
| 34 | AUTISM INITIATIVES GROUP | 2,881 | £93.1m |
| 35 | LEONARD CHESHIRE DISABILITY | 2,606 | £141m |
| 36 | United Church Schools Trust | 2,581 | £185m |
| 37 | THE ENGLISH HERITAGE TRUST | 2,548 | £155m |
| 38 | ROYAL SOCIETY FOR THE PROTECTION OF BIRDS | 2,486 | £195m |
| 39 | SUE RYDER | 2,462 | £106m |
| 40 | WELLCOME TRUST | 2,377 | £569m |
| 41 | AUTISM INITIATIVES (UK) | 2,316 | £72.6m |
| 42 | THE NATIONAL AUTISTIC SOCIETY | 2,265 | £102m |
| 43 | HCT GROUP | 2,225 | £124m |
| 44 | AFFINITY TRUST | 2,162 | £72.4m |
| 45 | UNIVERSITY OF SOUTH WALES/PRIFYSGOL DE CYMRU | 2,129 | £225m |
| 46 | UNIVERSITY OF WALES:TRINITY SAINT DAVID | 2,120 | £210m |
| 47 | MACINTYRE CARE | 2,061 | £71.2m |
| 48 | THE SALVATION ARMY SOCIAL WORK TRUST | 2,016 | £240m |
| 49 | METHODIST INDEPENDENT SCHOOLS TRUST | 1,977 | £118m |
| 50 | REAL LIFE OPTIONS | 1,949 | £55.6m |
| 51 | THE AVENUES TRUST GROUP | 1,945 | £67.2m |
| 52 | DOGS TRUST | 1,943 | £143m |
| 53 | HF TRUST LIMITED | 1,900 | £85.0m |
| 54 | ALZHEIMER'S SOCIETY | 1,896 | £144m |
| 55 | FUSION LIFESTYLE | 1,865 | £5.1m |
| 56 | THE ROYAL NATIONAL LIFEBOAT INSTITUTION | 1,859 | £251m |
| 57 | THE GUIDE DOGS FOR THE BLIND ASSOCIATION | 1,847 | £156m |
| 58 | GREENSLEEVES HOMES TRUST | 1,845 | £89.1m |
| 59 | DOGS TRUST LEGACY | 1,815 | £67.3m |
| 60 | CANAL & RIVER TRUST | 1,805 | £233m |
| 61 | THE DISABILITIES TRUST | 1,800 | £74.9m |
| 62 | AQA EDUCATION | 1,798 | £280m |
| 63 | ROYAL SOCIETY FOR THE PREVENTION OF CRUELTY TO ANIMALS | 1,791 | £153m |
| 64 | MACMILLAN CANCER SUPPORT | 1,782 | £246m |
| 65 | THE ROYAL BRITISH LEGION | 1,765 | £168m |
| 66 | THE PEOPLE'S DISPENSARY FOR SICK ANIMALS | 1,760 | £129m |
| 67 | FITZROY SUPPORT | 1,748 | £58.8m |
| 68 | THE BRANDON TRUST | 1,715 | £77.6m |
| 69 | GENERAL MEDICAL COUNCIL | 1,713 | £168m |
| 70 | NCFE | 1,709 | £49.5m |
| 71 | ST MUNGO COMMUNITY HOUSING ASSOCIATION | 1,682 | £124m |
| 72 | We Are With You | 1,670 | £87.0m |
| 73 | CARDIFF METROPOLITAN UNIVERSITY | 1,660 | £149m |
| 74 | ALTERNATIVE FUTURES GROUP LIMITED | 1,638 | £80.7m |
| 75 | THE SALVATION ARMY | 1,610 | £338m |
| 76 | BANGOR UNIVERSITY | 1,597 | £160m |
| 77 | CANTERBURY CHRIST CHURCH UNIVERSITY | 1,574 | £354m |
| 78 | THE CITY AND GUILDS OF LONDON INSTITUTE | 1,506 | £175m |
| 79 | ELIM FOURSQUARE GOSPEL ALLIANCE | 1,497 | £83.9m |
| 80 | THE NATIONAL SOCIETY FOR THE PREVENTION OF CRUELTY TO CHILDREN | 1,484 | £120m |
| 81 | BH LIVE | 1,483 | £48.1m |
| 82 | THE FRANCIS CRICK INSTITUTE LIMITED | 1,458 | £246m |
| 83 | UNIVERSITY OF CHESTER | 1,430 | £154m |
| 84 | THE PRIORY OF ENGLAND AND THE ISLANDS OF THE MOST VENERABLE ORDER OF THE HOSPITAL OF ST. JOHN OF JERUSALEM | 1,418 | £107m |
| 85 | THE FREMANTLE TRUST | 1,411 | £49.5m |
| 86 | ST MONICA TRUST | 1,392 | £53.7m |
| 87 | JISC | 1,382 | £151m |
| 88 | RUSKIN MILL TRUST LIMITED | 1,374 | £74.4m |
| 89 | DEPAUL INTERNATIONAL | 1,338 | £73.5m |
| 90 | PRIFYSGOL ABERYSTWYTH | 1,319 | £127m |
| 91 | AGE UK | 1,317 | £132m |
| 92 | GENOME RESEARCH LIMITED | 1,301 | £180m |
| 93 | TRUSTEES OF THE LONDON CLINIC LIMITED | 1,287 | £188m |
| 94 | THE ROYAL NATIONAL INSTITUTE OF BLIND PEOPLE | 1,286 | £95.9m |
| 95 | JEWISH CARE | 1,279 | £81.8m |
| 96 | THE EXTRACARE CHARITABLE TRUST | 1,278 | £69.9m |
| 97 | NATIONAL STAR FOUNDATION | 1,269 | £49.6m |
| 98 | THE ROYAL HORTICULTURAL SOCIETY | 1,267 | £125m |
| 99 | NURSING AND MIDWIFERY COUNCIL | 1,251 | £109m |
| 100 | VICTIM SUPPORT | 1,234 | £52.7m |
